I realize it may be a safer narrative for pro-FF pundits to accept the PR release from CFIA communications branch that there was no ISAv found in BC - but ISAv has been found in numerous samples from numerous geographic areas in BC numerous times using PCR testing, BN. I've posted some of those test results before on other threads. Those results include:
Cultus Lake - 64/64 sockeye smolts tested positive for ISA. Simon Jones (PBS Nanaimo) buried the results from the Cohen Comission and blocked Molly Kibenge from publishing on this. Cultus Lake sockeye got on the COSEWIC listing, but the Fisheries Minister blocked it from SARA due to economic impacts. No retesting nor no notification to the local FN. NO FOLLOW-UP! SFU also found ISA in local resident trout. Population remains depressed.
Oweekeno Lake - similar stock crash as Cultus. 6 Sockeye fry found with ISA. Samples were degraded and frozen which kills virus - but still got ISA positives. High prob of false negatives. Media scrum by DFO/CFIA. "We are winning the PR war" from CFIA emails in Cohen exhibits. Refusal of acceptance of ISA results by CFIA to protect the disease staus of the open net-cage industry. Again - no retesting or follow-up by DFO/CFIA. Instead they pay $2M to the OIE and get the only independent ISA lab in Charlottetown (that reported the presumptive positives) de-certified.
Southern NFLD and Inner Bay of Fundy Atlantic salmon stocks - all on SARA endangered list (IBoF) or listed to go on it (S. NFLD). All these stocks have been exposed to ISA from the net cage industry.
Because of the trade implications of those test results - CFIA made a decision to claim those test results were "false" verses "weak" positives - as they arbitrarily determined the cycles used to determine that weak/false determination. Then they gave something like $2M to the OIE and got Fred's lab decertified to make it all go away - and never retested and destroyed the samples. It's quite a story that probably deserves a movie to be made about it.
The ISAv (& PRv) sequences found in BC were European/Norwegian at one time, as well.
Molly JT Kibenge, Tokinori Iwamoto, Yingwei Wang, Alexandra Morton, Richard Routledge, and Frederick SB Kibenge. 2016. Discovery of variant infectious salmon anaemia virus (ISAV) of European genotype in British Columbia, Canada. Virology Journal (2016) 13:3
p.5: “ISAV sequences detected in British Columbia fish include both ISAV-HPRΔ and ISAV-HPR0 and are of European genotype”.
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Molly JT Kibenge, Tokinori Iwamoto, Yingwei Wang, Alexandra Morton, Marcos G Godoy, and Frederick SB Kibenge. 2013. Whole-genome analysis of piscine reovirus (PRV) shows PRV represents a new genus in family Reoviridae and its genome segment S1 sequences group it into two separate sub-genotypes. Virology Journal 2013, 10:230
p.10: “Chilean PRV strains had 100% amino acid sequence identity with the Norwegian strain Reovirus sp. Salmo/GP-2010/NOR, whereas the Canadian strains had ≤92.7% amino acid sequence identity with this [Norwegian] PRV strain…”
p.11: “Our analysis using BEAST simulation [32] shows the time when Canadian PRV isolates diverged from Norwegian PRV isolates was between 2006 and 2011”