Thanks for posting this Englishman. Forum members please read this very important letter.
We need to make phone calls, write letters to politicians and media, support anti net pen fish farming groups, or form our own concerned citizens groups etc., to pressure the Fed. Govt. to act to find out what the true and complete status is regarding this deadly virus NOW!
IF WE WAIT TOO LONG IT CAN SPREAD FURTHER AND POSSIBLY MUTATE TO OTHER SPECIES AND WIPE OUT ALL SALMON PERIOD!
NOW IS THE TIME TO DEMAND BETTER AND MORE ACTION. PLEASE DO SOMETHING ABOUT THIS ISSUE IF YOU CARE ABOUT FUTURE OF SALMON AND SALMON FISHING!!! IT IS TIME TO FOR US ALL TO WAKE UP AND DO SOMETHING!!!
It appears, it may have already "mutated"!
Conclusion
The RNA from both tissue types (gills and heart) seem to be of reasonable quality for real
time RT PCR, and the amount of RNA obtained after extraction was substantial. Hence, the
amount and quality of the RNA should not have influenced on the results.
Under ideal conditions we should be able to repeat all positive results when the Ct values are
below 37. The result obtained from sample H10 (gill tissues) was Ct = 34.5 and the result was
repeated once (Ct = 35.4), while the other four reruns were negative. The heart tissue from the
same individual was also negative. The gills from sample H14 was negative, but one of five
runs from the heart tissue was positive. None of the samples were positive when using the
Uni-ISAV8 assay. As can bee seen from the positive controls both assay have the same
sensitivity for detection of ISA virus RNA from European ISA viruses. This fact raises the
question:
What are we detecting with the ISA7 assay? Based on my experience with both
assays a reasonable answer to this question is that we are not detecting any of the known ISA
viruses from Europe (or from eastern North America). A more exact answer requires that we
are able to sequence the RNA that is target by the ISAV7 assay.
http://alexandramorton.typepad.com/Report231111%5B13%5D.pdf